Megazyme Sucrose/D-Fructose/D-Glucose Assay Kit

SKU: K-SUFRG

The Sucrose/D-Fructose/D-Glucose test kit is suitable for the measurement and analysis of sucrose, D-glucose and D-fructose in plant and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

 

UV-method for the determination of Sucrose, D-Fructose and
D-Glucose in foodstuffs, beverages and other materials

Principle:
(β-fructosidase)
(1) Sucrose + H2O → D-glucose + D-fructose

(hexokinase)
(2) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(3) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(5) F-6-P          →          G-6-P

 

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Stabilised D-glucose / D-fructose standard solution included
  • Extended cofactors stability
Kit Size

300 assays (100 of each) per kit

Method

Spectrophotometric at 340nm

Reaction Time

~ 20min

Detection Limit

1.38mg/L

Application Examples

Beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, dairy products, candies, desserts, confectionery, sweets, ice-cream, fruit and vegetables (e.g. potato), meat products (e.g. sausage), condiments (e.g. ketchup and mustard), feed, tobacco, cosmetics, pharmaceuticals, paper and other materials

Method Recognition

Methods based on this principle have been accepted by NF, EN, NEN, DIN, GOST, IFU, AIJN, MEBAK and IOCCC

Megazyme Sucrose/D-Fructose/D-Glucose Assay Kit (K-SUFRG) Data Booklet

Download