Megazyme Total Starch HK Assay Kit, 100 assays

SKU: K-TSHK

A modification of AOAC Method 996.11, AACC Method 76-13.01 and RACI Standard Method for the measurement, and analysis of total starch in cereal flours and food products. This kit contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0). The method has been further modified by adjusting the D-glucose determination to a hexokinase/glucose-6-phosphate dehydrogenase/NADP+ based format.

 

UV-method for the determination of Total Starch in grains,
animal feeds, foodstuffs and other materials

Principle:
(α-amylase, 100°C + DMSO)
(1) Starch granules + H2O → maltodextrins

(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose

(hexokinase)
(3) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(4) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

 

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
Kit Size

100 assays

Method

Spectrophotometric at 340nm

Total Assay Time

~ 90 min

Detection Limit

1-100% of sample weight

Application Examples

Cereal flours, food products and other materials

Method Recognition

AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard
Method No. 168) and RACI (Standard Method)

Megazyme Total Starch HK Assay Kit (K-TSHK) Data Booklet

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