Megazyme endo-Polygalacturonanase (Pectobacterium carotovorum)

SKU: E-PGALPC

High purity endo-Polygalacturonanase (Pectobacterium carotovorum) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Content:	5,000 Units
Shipping Temperature:	Ambient
Storage Temperature:	2-8^oC
Formulation:	In 3.2 M ammonium sulphate
Physical Form:	Suspension
Stability:	> 4 years at 4^oC

Specification
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Enzyme Activity	endo-Polygalacturonase
EC Number	3.2.1.15
CAZy Family	GH28
CAS Number	9032-75-1
Synonyms	polygalacturonase; (1→4)-alpha-D-galacturonan glycanohydrolase
Source	Pectobacterium carotovorum
Concentration	Supplied at ~ 14,000 U/mL
Expression	Recombinant from Pectobacterium carotovorum
Specificity	Random hydrolysis of α-1,4-D-galactosiduronic linkages in pectate and polygalacturonans.
Specific Activity	~ 540 U/mg (40oC, pH 5.5 on polygalacturonic acid)
Unit Definition	One Unit of endo-polygalacturonanase activity is defined as the amount of enzyme required to release one μmole of galacturonic acid from polygalacturonic acid (5 mg/mL) per min in sodium acetate buffer (100 mM), pH 5.5 at 40oC.
Temperature Optima	50oC
pH Optima	6
Application examples	Applications in carbohydrate research and in the food industry.