Megazyme Protease Subtilisin A (from Bacillus licheniformis) Powder

SKU: E-BSPRPD

High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.4.21.62
CAS: 9014-01-1

subtilisin; subtilisin A

Highly purified. From Bacillus licheniformis.
In p
owder form. For use in Megazyme Total Dietary Fiber test method.

Specific activity:
> 10 U/mg of protein; (40oC, pH 8.0 on casein).

Stability: Minimum 1 year at < -10oC. Check vial for details.

Content/size

1 gram

Synonyms

subtilisin; subtilisin A

Form

In powder form.

Stability

Minimum 1 year at < -10oC. Check vial for details

Specific activity

> 10 U/mg of protein; (40oC, pH 8.0 on casein)

Unit definition

One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 μg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent)

Specificity

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides

Applications

This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fibre analytical procedures

Megazyme Protease Subtilisin A (from Bacillus licheniformis) Powder (E-BSPRPD) Data Booklet

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