Megazyme Sucrase (from yeast)

SKU: E-SUCR

High purity Sucrase (from yeast) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. Sucrase and Fructanase (E-FRMXLQ) are used in the measurement of fructan in foods according to Chinese GB Standard 5009.255-2016.

EC 3.2.1.20
CAZy Family: GH13
CAS: 9001-42-7 

alpha-glucosidase; alpha-D-glucoside glucohydrolase

Highly purified. From yeast.
Lyophilised powder.

Specific activity:
~ 20 U/mg (40oC, pH 6.8 on sucrose).

Stability: Minimum 1 year at < -10oC. Check vial for details.

Content/size

300 Units (on sucrose)
Synonyms

alpha-glucosidase; alpha-D-glucoside glucohydrolase
Form

Lyophilised powder
Stability

Minimum 1 year at < -10oC. Check vial for details.
Specific activity

~ 20 U/mg (40oC, pH 6.8 on sucrose)
Unit definition

One Unit of sucrase activity is defined as the amount of enzyme required to release one μmole of glucose per minute from sucrose (10 mM) in sodium maleate buffer (100 mM), pH 6.8 at 40oC
Specificity

Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose.
Applications

Applications for the removal of sucrose in various analytical procedures in the cereals, food and feeds, fermentation and beverage industries

Megazyme Sucrase (from yeast) (E-SUCR) Data Booklet

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