Megazyme Total Dietary Fiber Assay Kit, 200 assays

SKU: K-TDFR-200A

The Total Dietary Fiber test kit is suitable for the measurement and analysis of Total Dietary Fiber in accordance with validated methods: AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01.

 

For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials

Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose

(protease)
(2) Protein + H2O → peptides

(3) Dietary fiber determined gravimetrically following alcohol
precipitation

(4) Ash and residual protein determined on DF residues
and subtracted

 

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years
  • High purity / standardised enzymes employed
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Simple format

PURCHASE ONLINE: K-TDFR-200A

Kit Size

200 assays

Method

Hydrolysis / removal of non-dietary fiber components

Total Assay Time

~ 100min

Detection Limit

0.5-100% of sample weight

Application Examples

Food ingredients, food products and other materials

Method Recognition

AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC
(Methods 32-05.01, 32-06.01, 32-07.01 and 32-21.01) and
CODEX (Type I Method)

Method 1

DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER

Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

Method 2

DETERMINATION OF TOTAL DIETARY FIBER

Based on AACC method 32-05.01 and AOAC Method 985.29.

Megazyme Total Dietary Fiber Assay Kit (K-TDFR) Data Booklet

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