| Enzyme Activity | Esterase |
|---|---|
| EC Number | 3.1.1.B11 |
| CAZy Family | CE15 |
| CAS Number | 9016-18-6 |
| Synonyms | glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase |
| Source | Ruminococcus flavefaciens |
| Molecular Weight | 47,000 |
| Concentration | Supplied at ~ 370 U/mL |
| Expression | Recombinant from Ruminococcus flavefaciens |
| Specificity | Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. |
| Specific Activity | ~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3) |
| Unit Definition | One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3. |
| Temperature Optima | 40oC |
| pH Optima | 7 |
| Application examples | Applications in carbohydrate research and in the food industry. |
Megazyme Glucuronoyl Esterase (Ruminococcus flavefaciens)
High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
| Content: | 250 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
