Megazyme β-Galactosidase (Aspergillus niger)

SKU: E-BGLAN

High purity β-Galactosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.23
CAZy Family: GH35
CAS: 9031-11-2

beta-galactosidase; beta-D-galactoside galactohydrolase

Highly purified. From Aspergillus niger.
In 3.2 M ammonium sulphate.
Supplied at ~ 4,000 U/mL.

Specific activity:
~ 200 U/mg (40oC, pH 4.5 on p-nitrophenyl β-D-galactoside).

Stability: > 4 years at 4oC.

Content/size

8,000 Units

Synonyms

beta-galactosidase; beta-D-galactoside galactohydrolase

Form

In 3.2 M ammonium sulphate

Stability

> 4 years at 4oC

Specific activity

~ 200 U/mg (40oC, pH 4.5 on p-nitrophenyl β-D-galactoside).

Unit definition

One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40oC

Specificity

Hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides

Applications

Applications established in diagnostics and research within the food and feed, carbohydrate and biofuels industries

Megazyme β-Galactosidase (Aspergillus niger) (E-BGLAN) Data Booklet

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