Megazyme D-Fructose/D-Glucose Assay Kit

SKU: K-FRUGL

D-Fructose/D-Glucose test kit, an enzymatic UV-method for the measurement and analysis of D-fructose and/or D-glucose in plant and food products.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

 

UV-method for the determination of D-Fructose and D-Glucose
in foodstuffs, beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(hexokinase)
(2) D-Fructose + ATP → F-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(3) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

(phosphoglucose isomerase)
(4) F-6-P             ↔             G-6-P

Kit size:    110 assays (manual) / 1100 (microplate)
/ 1100 (auto-analyser)

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave
Spectrophotometer (D-MQWAVE)
.

 

Advantages

  • PVP incorporated to prevent tannin inhibition
  • Validated by the University of Wine, Suze la Rousse, France
  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation (manual analysis applications)
  • Rapid reaction at either 25 or 37°C
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats
Kit Size

110 assays (manual) / 1100 (microplate) / 1100 (auto-analyser)

Method

Spectrophotometric at 340nm

Reaction Time

~ 13min

Detection Limit

0.66mg/L

Application Examples

Wine, beer, fruit juices, soft drinks, milk, jam, honey, dietetic foods, bread, bakery products, candies, desserts, confectionery, ice-cream, fruit and vegetables, condiments, tobacco, cosmetics, pharmaceuticals, paper and other materials (e.g. biological cultures, samples, etc.)

Method Recognition

Methods based on this principle have been accepted by AOAC, EN,
NEN, NF, DIN, GOST, OIV, IFU, AIJN, MEBAK and IOCCC

Megazyme D-Fructose/D-Glucose Assay Kit (K-FRUGL) Data Booklet

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