| Kit Size | 100 assays |
|---|---|
| Method | Hydrolysis / removal of non-dietary fiber components |
| Total Assay Time | ~ 100min |
| Detection Limit | 0.5-100% of sample weight |
| Application Examples | Food ingredients, food products and other materials |
| Method Recognition | AOAC (Methods 985.29, 991.42, 991.43 and 993.19), AACC |
| Method 1 | DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91). |
| Method 2 | DETERMINATION OF TOTAL DIETARY FIBER Based on AACC method 32-05.01 and AOAC Method 985.29. |
Megazyme Total Dietary Fiber Assay Kit, 100 assays
The Total Dietary Fiber test kit is suitable for the measurement and analysis of Total Dietary Fiber in accordance with validated methods: AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01.
For the determination of Total Dietary Fiber in cereal products,
foodstuffs, feeds and other materials
Principle:
(α-amylase + amyloglucosidase)
(1) Starch + H2O → D-glucose
(protease)
(2) Protein + H2O → peptides
(3) Dietary fiber determined gravimetrically following alcohol
precipitation
(4) Ash and residual protein determined on DF residues
and subtracted
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 2 years
- High purity / standardised enzymes employed
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Simple format
