Megazyme D-Glucose HK Assay Kit, 110 assays

SKU: K-GLUHK-110A

The D-Glucose HK (Regular) test kit is a high purity reagent for the measurement and analysis of D-glucose in plant and food products. Can be used in combination with other Megazyme’s products that require glucose determination.

Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.

Suitable for manual, auto-analyser and microplate formats.

 

UV-method for the determination of D-Glucose in foodstuffs,
beverages and other materials

Principle:
(hexokinase)
(1) D-Glucose + ATP → G-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(2) G-6-P + NADP+ → gluconate-6-phosphate + NADPH + H+

The number of manual tests per kit can be doubled if all volumes are halved.
This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).

 

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years after preparation
  • Rapid reaction
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Extended cofactors stability
  • Suitable for manual, microplate and auto-analyser formats
Kit Size

110 assays (manual) / 1100 (microplate) / 1000 (auto-analyser)

Method

Spectrophotometric at 340nm

Reaction Time

~ 5min

Detection Limit

0.66mg/L

Application Examples

Wine, beer, fruit juices, soft drinks, milk, jam, dietetic foods, bakery products, candies, fruit and vegetables, tobacco, cosmetics, pharmaceuticals (e.g. infusions), feed, paper (and cardboard) and other materials (e.g. biological cultures, samples, etc.)

Method Recognition

Methods based on this principle have been accepted by AOAC, EN, NEN, NF, DIN, GOST, OIV, IFU, AIJN and MEBAK

Megazyme D-Glucose HK Assay Kit (K-GLUCHK) Data Booklet

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