| Enzyme Activity | Fructanase |
|---|---|
| EC Number | endo-Inulinase: 3.2.1.7, |
| CAZy Family | GH32 |
| CAS Number | 9001-57-4, |
| Synonyms | endo-inulinase: 1-beta-D-fructan fructanohydrolase |
| Source | Aspergillus niger |
| Concentration | endo-inulinase: Supplied at ~ 100 U/mL |
| Expression | Recombinant from Aspergillus niger |
| Specificity | endo-Inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin. |
| Unit Definition | endo-Inulinase: One Unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4.5. |
| Temperature Optima | 60oC |
| pH Optima | 4.5 |
| Application examples | Applications in carbohydrate research and in the food industry. |
| Method recognition | AOAC Method 997.08 and AOAC Method 2000.11 |
Megazyme Fructanase Mixture (Ultrapure, recombinant, powder)
Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Components: exo-Inulinase (20,000 U/mL on kestose, at 40oC) and endo-inulinase (100 U/mL on inulin at 40oC).
Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan (AOAC Method 997.08) and in the measurement of Polydextrose (AOAC Method 2000.11).
| Content: | 20,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | Supplied as a freeze-dried powder |
| Physical Form: | Powder |
| Stability: | Minimum 1 year at < -10oC. Check vial for details. |
