Megazyme Pullulanase M1 (Klebsiella planticola)

SKU: E-PULKP

High purity Pullulanase M1 (Klebsiella planticola) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7 

pullulanase; pullulan 6-alpha-glucanohydrolase

Highly purified. From Klebsiella planticola. Electrophoretically homogeneous.
In 3.2 M ammonium sulphate.
Supplied at ~ 700 U/mL.

Specific activity:
~ 30 U/mg (40oC, pH 5.0 on pullulan).

Stability: > 4 years at 4oC.

Recommended pullulanase for research on starch structure.

Content/size

700 Units

Synonyms

pullulanase; pullulan 6-alpha-glucanohydrolase

Form

In 3.2 M ammonium sulphate

Stability

> 4 years at 4oC

Specific activity

~ 30 U/mg (40oC, pH 5.0 on pullulan)

Unit definition

One Unit of pullulanase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC

Specificity

Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α- and β-limit dextrins of amylopectin and glycogen

Applications

Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups

Megazyme Pullulanase M1 (Klebsiella planticola) (E-PULKP) Data Booklet

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