Megazyme Pullulanase M2 (Bacillus licheniformis)

SKU: E-PULBL

High purity Pullulanase M2 (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7

pullulanase; pullulan 6-alpha-glucanohydrolase

Highly purified. From Bacillus licheniformisElectrophoretically homogeneous (MW 113,000).
In 3.2 M ammonium sulphate.
Supplied at ~ 900 U/mL.

Specific activity:
~ 40 U/mg (40oC, pH 5.0 on pullulan).

StabilityMinimum 1 year at 4oC. Check vial for details.

Content/size

2,000 Units

Synonyms

pullulanase; pullulan 6-alpha-glucanohydrolase

Form

In 3.2 M ammonium sulphate

Stability

Minimum 1 year at 4oC. Check vial for details

Specific activity

~ 40 U/mg (40oC, pH 5.0 on pullulan)

Unit definition

One Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC

Specificity

Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α and β-limit dextrins of amylopectin and glycogen

Applications

Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups

Megazyme Pullulanase M2 (Bacillus licheniformis) (E-PULBL) Data Booklet

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