| Content/size | 2,000 Units |
|---|---|
| Synonyms | pullulanase; pullulan 6-alpha-glucanohydrolase |
| Form | In 3.2 M ammonium sulphate |
| Stability | Minimum 1 year at 4oC. Check vial for details |
| Specific activity | ~ 40 U/mg (40oC, pH 5.0 on pullulan) |
| Unit definition | One Unit of pullulanase M2 activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar-equivalents per minute from pullulan (5 mg/mL) in sodium acetate buffer (100 mM), pH 5.0 at 40oC |
| Specificity | Hydrolysis of (1,6)-α-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the α and β-limit dextrins of amylopectin and glycogen |
| Applications | Applications in the cereals, food and feeds industries particularly in starch saccharification and production of high glucose or maltose syrups |
Megazyme Pullulanase M2 (Bacillus licheniformis)
High purity Pullulanase M2 (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
EC 3.2.1.41
CAZy Family: GH13
CAS: 9075-68-7
pullulanase; pullulan 6-alpha-glucanohydrolase
Highly purified. From Bacillus licheniformis. Electrophoretically homogeneous (MW 113,000).
In 3.2 M ammonium sulphate.
Supplied at ~ 900 U/mL.
Specific activity:
~ 40 U/mg (40oC, pH 5.0 on pullulan).
Stability: Minimum 1 year at 4oC. Check vial for details.
