Enzyme Activity | Esterase |
---|---|
EC Number | 3.1.1.B11 |
CAZy Family | CE15 |
CAS Number | 9016-18-6 |
Synonyms | glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase |
Source | Ruminococcus flavefaciens |
Molecular Weight | 47,000 |
Concentration | Supplied at ~ 370 U/mL |
Expression | Recombinant from Ruminococcus flavefaciens |
Specificity | Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin. |
Specific Activity | ~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3) |
Unit Definition | One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3. |
Temperature Optima | 40oC |
pH Optima | 7 |
Application examples | Applications in carbohydrate research and in the food industry. |
Megazyme Glucuronoyl Esterase (Ruminococcus flavefaciens)
High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Content: | 250 Units |
Shipping Temperature: | Ambient |
Storage Temperature: | 2-8oC |
Formulation: | In 3.2 M ammonium sulphate |
Physical Form: | Suspension |
Stability: | > 4 years at 4oC |