Megazyme Glucuronoyl Esterase (Ruminococcus flavefaciens)

SKU: E-GERF

High purity Glucuronoyl Esterase (Ruminococcus flavefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

Content: 250 Units
Shipping Temperature: Ambient
Storage Temperature: 2-8oC
Formulation: In 3.2 M ammonium sulphate
Physical Form: Suspension
Stability: > 4 years at 4oC
Enzyme Activity

Esterase

EC Number

3.1.1.B11

CAZy Family

CE15

CAS Number

9016-18-6

Synonyms

glucuronoyl esterase, 4-O-methyl-glucuronoyl methylesterase

Source

Ruminococcus flavefaciens

Molecular Weight

47,000

Concentration

Supplied at ~ 370 U/mL

Expression

Recombinant from Ruminococcus flavefaciens

Specificity

Glucuronoyl esterase is believed to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin.

Specific Activity

~ 27 U/mg (40oC, pH 6.5 on pNP-GEUX3)

Unit Definition

One Unit of glucuronoyl esterase activity is defined as the amount of enzyme required to release one μmole of of p-nitrophenol (pNP) per minute from p-nitrophenyl-22-(methyl 4-O-methyl-α-D-glucopyranosyluronate)-β-D-xylobioside (5 mM) in sodium phosphate buffer (100 mM), pH 6.5 at 40oC. Analysis was carried out using the Glucuronoyl Esterase Assay kit, K-GEUX3.

Temperature Optima

40oC

pH Optima

7

Application examples

Applications in carbohydrate research and in the food industry.

Megazyme Glucuronoyl Esterase (Ruminococcus flavefaciens) Data Booklet

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