Megazyme Protease (Subtilisin A from Bacillus licheniformis), 40mL

SKU: E-BSPRT-40ML

High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.4.21.62
CAS: 9014-01-1 

subtilisin; subtilisin A

Highly purified. From Bacillus licheniformis.
In 50% (v/v) glycerol.

For use in Megazyme Total Dietary Fiber test method.

E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.

Specific activity:
~ 6 U/mg of protein (40oC, pH 8.0 on casein).

Stability: > 4 years at 4oC.

Content/size

2 grams – 40ML

Synonyms

subtilisin; subtilisin A

Form

In 50% (v/v) glycerol

Stability

> 4 years at 4oC

Specific activity

~ 6 U/mg of protein (40oC, pH 8.0 on casein)

Unit definition

One Unit will hydrolyse casein to produce colour equivalent to one µmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent)

Specificity

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides

Applications

This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fibre analytical procedures

Megazyme Protease (Subtilisin A from Bacillus licheniformis) (E-BSPRT) Data Booklet

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