Kit Size | 100 assays |
---|---|
Method | Spectrophotometric at 510nm |
Total Assay Time | ~ 90 min |
Detection Limit | 1-100% of sample weight |
Application Examples | Cereal flours, food products and other materials |
Method Recognition | AOAC (Method 996.11), AACC (Method 76-13.01), ICC (Standard Method |
Megazyme Total Starch Assay Kit (AA/AMG), 100 assays
The Total Starch (AA/AMG) test kit is used for the measurement and analysis of total starch in cereal flours and food products. This kit now contains an improved α-amylase that allows the amylase incubations to be performed at pH 5.0 (as well as pH 7.0).
Colourimetric method for the determination of Total Starch in
cereal products, feeds, foodstuffs and other materials
Principle:
(α-amylase, 100°C ± DMSO)
(1) Starch granules + H2O → maltodextrins
(amyloglucosidase)
(2) Maltodextrins + H2O → D-glucose
(glucose oxidase)
(3) D-Glucose + H2O + O2 → D-gluconate + H2O2
(peroxidase)
(4) 2H2O2 + p-hydroxybenzoic acid + 4-aminoantipyrine →
quinoneimine + 4H2O
Advantages
- Very competitive price (cost per test)
- All reagents stable for > 12 months after preparation
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included