Megazyme Fructanase Mixture (purified-liquid), 10mL

SKU: E-FRMXLQ

High purity Fructanase Mixture (purified-liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

For Fructan Determination (Liquid).

Fructanase, Amyloglucosidase (E-AMGDF) and Isoamylase (E-ISAMY) are used in the enzyme hydrolysis step of the AOAC method 2001.11 for the determination of polydextrose (a low molar mass dietary fiber) in foods.

EC 3.2.1.7
CAZy Family: GH32 

EC 3.2.1.80
CAZy Family: GH32 

Components: exo-inulinase 2,000 U/ml (on kestose, at 40oC), endo-inulinase ˜ 100 U/ml (on fructan at 40oC), α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40oC), β-Glucanase < 0.04 U/ml (on β-glucan at 40oC) and pectinase < 0.07 U/ml (on pectin at 40oC).

Note: This product has been purified to remove α-galactosidase, β-glucanase and pectinase which interfere with the use of the preparation in the measurement of fructan, or in the solubilisation of “insoluble” fructan in the AOAC Total Dietary Fiber method. In this product, the contamination of exo-inulinase by α-galactosidase, β-glucanase and pectinase is 0.004%, 0.002% and 0.03%, respectively. In Fructozyme (NOVO), the contamination by α-galactosidase, β-glucanase and pectinase is 15.2%, 0.3% and 2% respectively.

Stability: Minimum 1 year at < -10oC. Check vial for details.

Content/size

10mL

Form

In 3.2 M ammonium sulphate

Stability

Minimum 1 year at < -10oC. Check vial for details.

Specific activity

exo-inulinase 2,000 U/ml (on kestose, at 40oC)
endo-inulinase ˜ 100 U/ml (on fructan at 40oC)
α-galactosidase < 0.08 U/ml (on polygalacturonic acid at 40oC)
β-Glucanase < 0.04 U/ml (on β-glucan at 40oC)
pectinase < 0.07 U/ml (on pectin at 40oC)

Megazyme Fructanase Mixture (purified-liquid) (E-FRMXLQ) Data Booklet

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